期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 82, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2020.108398
关键词
L-Theanine; Vascular smooth muscle cell; Neointimal hyperplasia; Phenotypic modulation; Elk-1
资金
- National Natural Science Foundation of China [31571166, 81602733, 81711703]
Neointimal hyperplasia is a prominent pathological phenomenon in the process of stent restenosis. Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) play major pathological processes involved in the development of restenosis. L-Theanine, one of the major amino acid components in green tea, has been reported to improve vascular function. Here we display the effects of L-theanine on neointima formation and the underlying mechanism. In the rat carotid-artery balloon-injury model, L-theanine greatly inhibited neointima formation and prevented VSMCs from a contractile phenotype switching to a synthetic phenotype. In vitro study showed that L-theanine significantly inhibited PDGF-BB-induced VSMC proliferation and migration, which was comparable with the effect of L-theanine on Angli-induced VSMC proliferation and migration. Western blot analysis demonstrated that L-theanine suppressed PDGF-BB and Angli-induced reduction of SMA and SM22 alpha and increment of OPN, suggesting that L-theanine inhibited the transformation of VSMCs from contractile to the synthetic phenotype. Further experiments showed that L-theanine exhibits potential preventive effects on neointimal hyperplasia and related vascular remodeling via inhibition of phosphorylation of Elk-1 and activation of MAPK1. The present study provides the new experimental evidence that L-theanine has potential clinical application as an anti-restenosis agent for the prevention of restenosis. (C) 2020 Elsevier Inc. All rights reserved.
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