期刊
JOURNAL OF NEUROSCIENCE
卷 40, 期 34, 页码 6503-6521出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3006-19.2020
关键词
astrocytes; development; microglia; neural-immune; vasculature
资金
- National Institute of Mental Health [R01-MH-113743]
- National Institute of Allergy and Infectious Diseases [T32-A1-095213]
- AHA Predoctoral Fellowship [19PRE3480616]
- Brain & Behavior Research Foundation
- Charles H. Hood Foundation
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- Deutsche Forschungsgemeinschaft (German Research Foundation) [390857198]
- National Institute of Neurological Disorders and Stroke Grant [P01-NS-083513]
Microglia, a resident CNS macrophage, are dynamic cells, constantly extending and retracting their processes as they contact and functionally regulate neurons and other glial cells. There is far less known about microglia-vascular interactions, particularly under healthy steady-state conditions. Here, we use the male and female mouse cerebral cortex to show that a higher percentage of microglia associate with the vasculature during the first week of postnatal development compared with older ages and that the timing of these associations is dependent on the fractalkine receptor (CX3CR1). Similar developmental microglia-vascular associations were detected in the human brain. Using live imaging in mice, we found that juxtavascular microglia migrated when microglia are actively colonizing the cortex and became stationary by adulthood to occupy the same vascular space for nearly 2 months. Further, juxtavascular microglia at all ages associate with vascular areas void of astrocyte endfeet, and the developmental shift in microglial migratory behavior along vessels corresponded to when astrocyte endfeet more fully ensheath vessels. Together, our data provide a comprehensive assessment of microglia-vascular interactions. They support a mechanism by which microglia use the vasculature to migrate within the developing brain parenchyma. This migration becomes restricted on the arrival of astrocyte endfeet such that juxtavascular microglia become highly stationary and stable in the mature cortex.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据