期刊
JOURNAL OF NANOMATERIALS
卷 2020, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2020/9346423
关键词
-
资金
- Priming Scientific Research Foundation
- Department of Science and Technology of Ho Chi Minh City [105/2017/HD-SKHCN]
Early and highly accurate detection of diverse diseases is in urgent demand than ever, especially for cancers and infectious ones. Among possibilities, biosensing by utilizing conjugated nanoparticles is still a method of choice. However, the toxicity of quantum dots remains a big matter of concern in those biooriented applications. In this study, mercaptosuccinic acid-coated cadmium selenide quantum dots of approximately 2.3 nm were synthesized with a simple green method at low temperature and cost-saving chemicals. The influence of synthesis factors was investigated with different spectroscopic methods. The toxicity issue was evaluated on the NIH-3T3 cell line (ATCC (R) CRL-1658 (TM)) and an MTT assay, revealing a secure threshold of 20 mu g/ml. Consequently, successful conjugation to the CD3 antibody including an A/G protein bridge was implemented and verified with fluorescent methods. Finally, Jurkat T cell detectability of conjugated CdSe was successfully validated with fluorescent microscopy. The CdSe-based products are accessible for future biosensing applications.
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