期刊
JOURNAL OF MOLECULAR MEDICINE-JMM
卷 98, 期 9, 页码 1301-1317出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00109-020-01954-3
关键词
Trichuris muris; B cells; IgMi mouse; Interleukin-10; Th1; Th2; Intestinal pathology
资金
- Indonesian endowment fund for education, PhD studentship
- Medical Research Council UK [MR/NO22661/1]
- Biotechnology and Biological Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/P018157/1]
The IgMi mouse has normal B cell development; its B cells express an IgM B cell receptor but cannot class switch or secrete antibody. Thus, the IgMi mouse offers a model system by which to dissect out antibody-dependent and antibody-independent B cell function. Here, we provide the first detailed characterisation of the IgMi mouse post-Trichuris muris(T. muris) infection, describing expulsion phenotype, cytokine production, gut pathology and changes in T regulatory cells, T follicular helper cells and germinal centre B cells, in addition to RNA sequencing (RNA seq) analyses of wild-type littermates (WT) and mutant B cells prior to and post infection. IgMi mice were susceptible to a high-dose infection, with reduced Th2 cytokines and elevated B cell-derived IL-10 in mesenteric lymph nodes (MLN) compared to controls. A low-dose infection regime revealed IgMi mice to have significantly more apoptotic cells in the gut compared to WT mice, but no change in intestinal inflammation. IL-10 levels were again elevated. Collectively, this study showcases the potential of the IgMi mouse as a tool for understanding B cell biology and suggests that the B cell plays both antibody-dependent and antibody-independent roles post high- and low-doseT. murisinfection. Key messages During a high-dose infection, B cells are important in maintaining the Th1/Th2 balance in the MLN through an antibody-independent mechanism. High levels of IL-10 in the MLN early post-infection, and the presence of IL-10-producing B cells, correlates with susceptibility to infection. B cells maintain gut homeostasis during chronic infection via an antibody-dependent mechanism.
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