Full-field strain of regenerated bone tissue in a femoral fracture model

The mechanical behaviour of regenerated bone tissue during fracture healing is key in determining its ability to withstand physiological loads. However, the strain distribution in the newly formed tissue and how this influences the way a fracture heals it is still unclear. X-ray Computed Tomography (XCT) has been extensively used to assess the progress of mineralised tissues in regeneration and when combined within situmechanics and digital volume correlation (DVC) has been proven a powerful tool to understand the mechanical behaviour and full-field three-dimensional (3D) strain distribution in bone. The purpose of this study is therefore to usein situXCT mechanics and DVC to investigate the strain distribution and load-bearing capacity in a regenerating fracture in the diaphyseal bone, using a rodent femoral fracture model stabilised by external fixation. Rat femurs with 1 mm and 2 mm osteotomy gaps were tested underin situXCT step-wise compression in the apparent elastic region. High strain was present in the newly formed bone (epsilon(p1)and epsilon(p3)reaching 29 000 mu epsilon and -43 000 mu epsilon, respectively), with a wide variation and inhomogeneity of the 3D strain distribution in the regenerating tissues of the fracture gap, which is directly related to the presence of unmineralised tissue observed in histological images. The outcomes of this study will contribute in understanding natural regenerative ability of bone and its mechanical behaviour under loading.

Automated summary

This study investigated the strain distribution and load-bearing capacity in regenerating fractures using in situ XCT mechanics and DVC. The results showed significant variation and inhomogeneity in the 3D strain distribution in the regenerating tissues of the fracture gap, which was associated with the presence of unmineralised tissue observed in histological images.