期刊
JOURNAL OF LIPID RESEARCH
卷 61, 期 11, 页码 1450-1463出版社
ELSEVIER
DOI: 10.1194/jlr.RA120001021
关键词
biosynthesis; metabolome; microbiome; proteomics; Oligo-MM12; fibroblast growth factor 15; Cyp7a1; Sult2a8; farnesoid X receptor
资金
- Swiss National Science Foundation Sinergia [CRSII3_147603, CRSII5_180317]
- Swiss National Science Foundation (SNF) [CRSII5_180317, CRSII3_147603] Funding Source: Swiss National Science Foundation (SNF)
Bile acids, which are synthesized from cholesterol by the liver, are chemically transformed along the intestinal tract by the gut microbiota, and the products of these transformations signal through host receptors, affecting overall host health. These transformations include bile acid deconjugation, oxidation, and 7 alpha-dehydroxylation. An understanding of the biogeography of bile acid transformations in the gut is critical because deconjugation is a prerequisite for 7 alpha-dehydroxylation and because most gut microorganisms harbor bile acid transformation capacity. Here, we used a coupled metabolomic and metaproteomic approach to probe in vivo activity of the gut microbial community in a gnotobiotic mouse model. Results revealed the involvement of Clostridium scindens in 7 alpha-dehydroxylation, of the genera Muribaculum and Bacteroides in deconjugation, and of six additional organisms in oxidation (the genera Clostridium, Muribaculum, Bacteroides, Bifidobacterium, Acutalibacter, and Akkermansia). Furthermore, the bile acid profile in mice with a more complex microbiota, a dysbiosed microbiota, or no microbiota was considered. For instance, conventional mice harbor a large diversity of bile acids, but treatment with an antibiotic such as clindamycin results in the complete inhibition of 7 alpha-dehydroxylation, underscoring the strong inhibition of organisms that are capable of carrying out this process by this compound. Finally, a comparison of the hepatic bile acid pool size as a function of microbiota revealed that a reduced microbiota affects host signaling but not necessarily bile acid synthesis. In this study, bile acid transformations were mapped to the associated active microorganisms, offering a systematic characterization of the relationship between microbiota and bile acid composition.
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