期刊
JOURNAL OF INFECTIOUS DISEASES
卷 223, 期 4, 页码 721-732出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiaa412
关键词
Listeria monocytogenes; BER; DNA glycosylase; AP endonuclease; DNA repair; virulence
资金
- Zhejiang University
This study highlights the importance of base excision repair (BER) for Listeria monocytogenes virulence and survival during host colonization. By deleting specific DNA glycosylases and apurinic/apyrimidinic endonucleases, researchers found that certain mutants were attenuated in virulence in a mouse model and showed reduced colonization in the liver and spleen.
Background. Base excision repair (BER), consisting mostly of lesion-specific DNA glycosylases and apurinic/apyrimidinic (AP) endonucleases, is one of the most important DNA repair mechanisms for repair of single nucleobase lesions generated by reactive oxygen and nitrogen species as part of an immune response against bacterial infections. However, few studies have addressed the contribution of BER to bacterial virulence and Listeria monocytogenes BER has thus far remained completely uncharacterized. Methods. Analysis of the L. monocytogenes EGDe genome identified 7 DNA glycosylases (MutM, MutY, Nth, Tag, Mpg, Ung, and Ung2) and 2 apurinic/apyrimidinic endonucleases (Xth and Nfo) as part of BER. Markerless in-frame deletion mutants were generated for all 9 genes, and mutants were tested for DNA damage survival, mutagenesis, and the ability to colonize a mouse model of infection. Results. Distinct lesion-specific phenotypes were identified for all deletion mutants. Importantly, the Delta nth, Delta mutY, and Delta nfo mutants were significantly attenuated for virulence in the mouse model and showed much lower colonization of the liver and spleen or were unable to compete with the wild-type strain during in vivo competition assays. Conclusions. Our results highlight the importance of BER for L. monocytogenes virulence and survival of DNA-damaging insults during host colonization.
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