期刊
JOURNAL OF INFECTIOUS DISEASES
卷 223, 期 5, 页码 848-853出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiaa449
关键词
chancre; China; syphilis; Treponema pallidum; whole-genome sequencing
资金
- Medical Scientific Research Foundation of Guangdong Province, China [A2018264, B2019022]
- Science and Technology Planning Project of Guangdong Province, China [2017A020212008]
- National Institute for Allergy and Infectious Diseases [U19AI144177, K24AI143471, K24AI134990, R01AI26756]
- Yang Biomedical Scholars Award
- Clara Guthrie Patterson Trust
- Bank of America, N.A., Trustee
- Connecticut Children's Medical Center
- Grant Agency of the Czech Republic [GA17-25455S]
- American Society for Tropical Medicine and Hygiene-Burroughs Wellcome Foundation Award
This study successfully developed a new method for direct TPA whole-genome sequencing from patient samples, overcoming previous limitations due to in vitro cultivation methods. Using this approach, researchers obtained high-quality genome data from Chinese samples and gained insights into the genetic diversity of TPA in China.
Background. Whole-genome sequencing (WGS) of lreponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. Methods. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. Conclusions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据