An expedient multi-component synthesis of pyridinyl-spirooxindoles and their effect on proliferation of lung cancerA549cells

A series of new functionalized pyridinyl-spirooxindoles have been synthesized through three-component cyclization reactions. The selected compounds were screened for their in vitro antiproliferative activity against human lung cancer cell line A549. Among the candidate structures, compound1odemonstrated maximum inhibitory activity against A549 cells with IC(50)values of 28.38 mu M. EdU (5-Ethynyl-2 '- deoxyuridine, EdU) assay and cell colony formation test showed that cell proliferation of A549 cells was inhibited. In addition, Western blot analysis revealed that the phosphorylation levels of Akt, mTOR, 70S6, and S6 were down-regulated. Thus, these results indicated that1omay inhibit the proliferation of A549 cells through inhibiting the phosphorylation levels of Akt, mTOR, 70S6, and S6.1omay be developed as a potential antitumor agent for lung cancer treatment.