期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 71, 期 20, 页码 6379-6395出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eraa368
关键词
Lignin biosynthesis; monolignol transport; plasma membrane; proteomics; transporter proteins
资金
- Viikki Doctoral Programme in Molecular Biosciences
- Jenny and Antti Wihuri Foundation
- Research Funds of the University of Helsinki
- Academy of Finland [251390, 256174, 283245]
- Faculty of Biological and Environmental Sciences
- Gun och Bertil Stohnes Stiftelse
- Adlerbertska Forskningsstiftelsen (Kungliga Vetenskaps-och Vitterhets-Samhallet)
- Syskonen Svenssons Fond for Medicinsk Forskning
- Integrative Life Science Doctoral Program
- Academy of Finland (AKA) [251390, 256174, 251390, 256174, 283245, 283245] Funding Source: Academy of Finland (AKA)
Both the mechanisms of monolignol transport and the transported form of monolignols in developing xylem of trees are unknown. We tested the hypothesis of an active, plasma membrane-localized transport of monolignol monomers, dimers, and/or glucosidic forms with membrane vesicles prepared from developing xylem and lignin-forming tissuecultured cells of Norway spruce (Picea abies L. Karst.), as well as from control materials, comprising non-lignifying Norway spruce phloem and tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem and BY-2 vesicles transported both coniferin and p-coumaryl alcohol glucoside, but inhibitor assays suggested that this transport was through the tonoplast. Membrane vesicles prepared from lignin-forming spruce cells showed coniferin transport, but the K-m value for coniferin was much higher than those of xylem and BY-2 cells. Liquid chromatography-mass spectrometry analysis of membrane proteins isolated from spruce developing xylem, phloem, and lignin-forming cultured cells revealed multiple transporters. These were compared with a transporter gene set obtained by a correlation analysis with a selected set of spruce monolignol biosynthesis genes. Biochemical membrane vesicle assays showed no support for ABC-transporter-mediated monolignol transport but point to a role for secondary active transporters (such as MFS or MATE transporters). In contrast, proteomic and co-expression analyses suggested a role for ABC transporters and MFS transporters.
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