期刊
JOURNAL OF ENVIRONMENTAL SCIENCES
卷 94, 期 -, 页码 161-170出版社
SCIENCE PRESS
DOI: 10.1016/j.jes.2020.03.052
关键词
Proteomics; Phytoremediation; Plant and bacteria interactions; Benzene; Air pollution
资金
- King Mongkut's University of Technology Thonburi
- KMUTT 55th Anniversary Commemorative Fund
Phytoremediation is a sustainable remedial approach for removing benzene from environment. Plant associated bacteria could ameliorate the phytotoxic effects of benzene on plant, although the specificity of these interactions is unclear. Here, we used proteomics approach to gain a better understanding of the mechanisms involved in plant-bacteria interactions. Plant associated bacteria was isolated and subsequently inoculated into the sterilized Helianthus annuus, and the uptake rates of benzene by these inoculated plants were evaluated. At the end of the experiment, leaves and roots proteins were analyzed. The results showed inoculated H. annuus with strain EnL3 removed more benzene than other treatments after 96 h. EnL3 was identified as Enterobacter sp. according to 16S rDNA analysis. Based on the comparison of proteins, 62 proteins were significantly up or down regulated in inoculated leaves, while 35 proteins were significantly up or down regulated in inoculated roots. Furthermore, there were 4 and 3 identified proteins presented only in inoculated H. annuus leaves and roots, respectively. These proteins involved in several functions including transcription and translation, photosynthesis, and stress response. The network among anti-oxidant defense system, protein synthesis, and photosynthetic electron transfer are involved in collaboratively activate the benzene uptake and stress tolerance in plant. (C) 2020 Published by Elsevier B.V. on behalf of The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据