期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1621, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.chroma.2020.461085
关键词
Electrokinetic chromatography; Amino acid based chiral ionic liquids; Cyclodextrin; Enantioseparation; Econazole; Sulconazole
资金
- Spanish Ministry of Economy and Competitiveness (MINECO) [CTQ2016-76368-P]
- Comunidad of Madrid
- FSE [S2018/BAA-4393]
- FEDER [S2018/BAA-4393]
- MINECO [RYC2013-12688]
- University of Alcala
Two analytical methodologies based on the combined use of hydroxypropyl-beta-cyclodextrin and two different amino acid-based chiral ionic liquids (tetrabutylammonium-L-lysine or tetrabutylammonium-L-glutamic acid) in electrokinetic chromatography were developed in this work to perform the enantios-elective determination of econazole and sulconazole in pharmaceutical formulations. The influence of different experimental variables such as buffer concentration, applied voltage, nature and concentration of the ionic liquid, temperature and injection time, on the enantiomeric separation was investigated. The combination of hydroxypropyl-beta-cyclodextrin and tetrabutylammonium-L-lysine under the optimized conditions enabled to achieve the enantiomeric determination of both drugs with high enantiomeric resolution (3.5 for econazole and 2.4 for sulconazole). The analytical characteristics of the developed methodologies were evaluated in terms of linearity, precision, LOD, LOQ and recovery showing good performance for the determination of both drugs which were successfully quantitated in pharmaceutical formulations. This work reports the first analytical methodology enabling the enantiomeric determination of sulconazole in pharmaceutical formulations. (C) 2020 Elsevier B.V. All rights reserved.
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