期刊
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
卷 147, 期 1, 页码 223-233出版社
SPRINGER
DOI: 10.1007/s00432-020-03325-4
关键词
Stem cell signatures; NSCLC; Early diagnosis; Survival predication
类别
资金
- National Science Foundation for Young Scientists of China [81602597]
- Foundation Research Project of Shaanxi Province (The Natural Science Fund) [2018JM7017]
The stem signatures associated antibodies MAGEA1, PGP9.5, SOX2, and TP53 showed high expressions in NSCLC, negatively correlating with overall survival. These antibodies exhibited high specificity and sensitivity in early diagnosis, presenting potential applications in clinical practice.
Background This study was designed to detect patients with early NSCLC with tentatively using the stem signatures associated autoantibodies (AAbs), and to evaluate its latent values in the early diagnosis and precise prognosis prediction. Methods The serum concentrations of selective antibodies were quantitated by enzyme-linked immunosorbent assay (ELISA), and a total of 458 cases were enrolled (training set = 401; validation set = 57). TCGA databases were used to analyze the distinct expressions and prognostic values of related genes. The optimal cut-off values were 11.60 U/ml for P53, 4.90 U/ml for MAGEA1, 3.85 U/ml for SOX2, and 7.05U/ml for PGP9.5. Results We found that the stem signatures associated antibodies of MAGEA1, PGP9.5, SOX2, and TP53 exhibited high expressions in NSCLC, negatively correlating with the overall survival (OS) (P < 0.05). In the test groups, the diagnosis sensitivity of P53, PGP9.5, SOX2, and MAGEA1 reached to 21.5%, 39.0%, 50.3%, and 35.0%, respectively, and the specificity reached to 98.7%, 99.4%, 92.2%, and 97.4%. The four candidates' panel gave a sensitivity of 71.8% with a specificity of 89%. In the validation group, the detection of the four antibodies in early diagnosis of NSCLC also exhibited high specificity and sensitivity, further consolidating their potential application. Conclusions The detection regarding stem signatures associated antibodies could be used as effective tools in early NSCLC diagnosis, but not for localized screening of cancers, and their abnormal expression was in accordance with poorer survival.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据