4.5 Article

Enhanced isobutanol production by co-production of polyhydroxybutyrate and cofactor engineering

期刊

JOURNAL OF BIOTECHNOLOGY
卷 320, 期 -, 页码 66-73

出版社

ELSEVIER
DOI: 10.1016/j.jbiotec.2020.06.017

关键词

Escherichia coli; Polyhydroxybutyrate; Isobutanol; Co-production; Acetate; Glucose utilization

资金

  1. National Research Foundation of Korea (NRF) [NRF-2019R1F1A1058805, NRF-2019M3E6A1103979]
  2. Research Program to solve social issues of the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2017M3A9E4077234]
  3. R&D Program of MOTIE/KEIT [20009508]

向作者/读者索取更多资源

Once cells have been used to produce biochemicals, there are only a few effective ways to utilize the residual cell mass, even though the utilization of leftover cells would aid in decreasing production costs. Here, a polyhydroxybutyrate (PHB) and isobutanol co-production system was designed to address this challenge. The addition of the PHB operon into Escherichia coli conferred a metabolic advantage for alcohol production, generating 1.14-fold more isobutanol. Furthermore, following nitrogen source optimization and cofactor engineering, the engineered E. coli strain produced 2-fold more isobutanol and 0.25 g/L PHB. Moreover, E. coli cells showed higher tolerance to isobutanol with the overexpression of PHB biosynthesis genes. This co-production system resulted in an increased biomass, higher glucose utilization, and lower acetate maintenance, leading to higher productivity regarding PHB and isobutanol yield. Thus, this novel system is applicable to future fermentation studies for the co-production of PHB and isobutanol.

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