期刊
JOURNAL OF BIOTECHNOLOGY
卷 320, 期 -, 页码 66-73出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2020.06.017
关键词
Escherichia coli; Polyhydroxybutyrate; Isobutanol; Co-production; Acetate; Glucose utilization
资金
- National Research Foundation of Korea (NRF) [NRF-2019R1F1A1058805, NRF-2019M3E6A1103979]
- Research Program to solve social issues of the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2017M3A9E4077234]
- R&D Program of MOTIE/KEIT [20009508]
Once cells have been used to produce biochemicals, there are only a few effective ways to utilize the residual cell mass, even though the utilization of leftover cells would aid in decreasing production costs. Here, a polyhydroxybutyrate (PHB) and isobutanol co-production system was designed to address this challenge. The addition of the PHB operon into Escherichia coli conferred a metabolic advantage for alcohol production, generating 1.14-fold more isobutanol. Furthermore, following nitrogen source optimization and cofactor engineering, the engineered E. coli strain produced 2-fold more isobutanol and 0.25 g/L PHB. Moreover, E. coli cells showed higher tolerance to isobutanol with the overexpression of PHB biosynthesis genes. This co-production system resulted in an increased biomass, higher glucose utilization, and lower acetate maintenance, leading to higher productivity regarding PHB and isobutanol yield. Thus, this novel system is applicable to future fermentation studies for the co-production of PHB and isobutanol.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据