4.7 Article

Study on the binding of sulfaclozine sodium monohydrate with bovine and human serum albumins using multi-spectroscopy and molecular docking

期刊

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
卷 39, 期 13, 页码 4835-4844

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2020.1780945

关键词

Sulfaclozine; sodium monohydrate; serum albumins; multi-spectroscopy; molecular docking

资金

  1. Natural Science Fund Project of Jilin Province Science and Technology Department [20170101020JC]

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The study investigated the interactions of sulfaclozine sodium monohydrate with bovine and human serum albumins using multi-spectroscopy and molecular docking techniques. The main force maintaining the stability of the compound was found to be electrostatic attraction based on thermodynamic parameters. Results from fluorescence measurements and molecular docking showed that SSM interacted with BSA/HSA predominantly at site I.
The interactions of sulfaclozine sodium monohydrate (SSM) with bovine and human serum albumins (BSA and HSA) were studied by multi-spectroscopy and molecular docking technique. Stern-Volmer analysis and fluorescence lifetime measurements suggested the quenching processes were static. According to the Fluorescence resonance energy transfer (FRET) theory, the binding distances were obtained indicating SSM interacted with BSA/HSA along with non-radiation energy conversion. Electrostatic attraction was the main force in keeping the stability of the compound based on thermodynamic parameters. Circular dichroism (CD), synchronous fluorescence and Fourier Transform infrared (FT-IR) spectra embodied the secondary structures of serum albumins were transformed by SSM. The site marker competitive and molecular docking measurements testified SSM bound to BSA/HSA at site I. In conclusion, the secondary structures of BSA/HSA were changed by SSM in the static fluorescence quenching processes with the non-radiation energy conversion. The binding sites were all located at site I and electrostatic attraction was the main force for the new compound. Communicated by Ramaswamy H. Sarma

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