期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 109, 期 4, 页码 515-523出版社
WILEY
DOI: 10.1002/jbm.a.37034
关键词
bone marrow-derived mesenchymal stem cells; bone regeneration; graphene; silk fibroin
资金
- National Natural Science Foundation of China [31771058, 31470938, 31771042, 11421202, 61227902, 11120101001]
- National Key Technology RD Program [2016YFC1100704, 2016YFC1101101]
- Fundamental Research Funds for the Central Universities
- International Joint Research Center of Aerospace Biotechnology and Medical Engineering from Ministry of Science and Technology of China
- 111 Project [B13003]
- Research Fund for the Doctoral Program of Higher Education of China [20131102130004]
By combining silk fibroin (SF) scaffolds with graphene, osteogenic scaffolds capable of accelerating bone formation were developed. Graphene affected the microarchitecture of the scaffolds, with the 0.5% graphene-doped SF scaffolds showing enhanced osteogenic properties. The results suggest that graphene-incorporated SF scaffolds could be a suitable option for bone tissue engineering.
Porous three-dimensional (3D) silk fibroin (SF) scaffolds were widely applied for bone regeneration and showed excellent biocompatibility and biodegradability. Recently graphene was developed for bone scaffolds due to its osteogenic properties. Thus, we combine the SF and graphene to improve the osteogenic properties of SF scaffolds. In our study, we explored the incorporation of SF scaffolds with graphene to develop osteogenic scaffolds capable of accelerating bone formation. The 3D SF scaffolds were fabricated with different contents of graphene (0, 0.5, and 2%). Fluorescence images showed that the graphene nanosheets were homogeneously dispersed in the SF scaffolds. The addition of graphene affected the microarchitecture of the scaffolds. The G/SF scaffolds were cocultured with rat bone marrow-derived mesenchymal stem cells (rBMSCs) for 21 days. The cell morphology and cell proliferation study suggested that 0 and 0.5% G/SF scaffolds displayed good cell proliferation. In addition, immunofluorescent staining (e.g., osteonectin, osteopontin, and osteocalcin) and ALP activities indicated that the osteogenic properties was more actively exhibited on 0.5% G/SF scaffolds compared with the other groups. Our results indicated that SF scaffolds incorporated with graphene could be an appropriate scaffold for bone tissue engineering.
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