期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 295, 期 32, 页码 11195-11213出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA120.013589
关键词
ribosomal RNA processing (rRNA processing); RNA degradation; nucleus; nucleolus; nuclear translocation; exosome complex
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [15/06477-9]
- Centre de Biologie Integrative, Toulouse
- CNPq [17/17777-9]
- FAPESP [17/17777-9]
- Research Internships Abroad Grant [18/19451-6]
- Centre National de la Recherche Scientifique
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [15/06477-9, 17/17777-9] Funding Source: FAPESP
The RNA exosome is a multisubunit protein complex involved in RNA surveillance of all classes of RNA, and is essential for pre-rRNA processing. The exosome is conserved throughout evolution, present in archaea and eukaryotes from yeast to humans, where it localizes to the nucleus and cytoplasm. The catalytically active subunit Rrp44/Dis3 of the exosome in budding yeast (Saccharomyces cerevisiae) is considered a protein present in these two subcellular compartments, and here we report that it not only localizes mainly to the nucleus, but is concentrated in the nucleolus, where the early pre-rRNA processing reactions take place. Moreover, we show by confocal microscopy analysis that the core exosome subunits Rrp41 and Rrp43 also localize largely to the nucleus and strongly accumulate in the nucleolus. These results shown here shed additional light on the localization of the yeast exosome and have implications regarding the main function of this RNase complex, which seems to be primarily in early pre-rRNA processing and surveillance.
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