期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 295, 期 40, 页码 13798-13811出版社
ELSEVIER
DOI: 10.1074/jbc.RA120.014976
关键词
lung cancer; proteasome degradation; ENH; STUB1; E3 ubiquitin ligase; transforming growth factor beta (TGF-B); lung cancer; cell invasion; cell motility; invasion; protein degradation; signal transduction; tumor metastasis; ubiquitin
资金
- Zhejiang Provincial Natural Science Foundation of China [LZ18H020001]
- National Natural Science Foundation of China [81673512, 31871399, 81530001, 81700064]
Transforming growth factor beta (TGF beta) signaling plays an important role in regulating tumor malignancy, including in non-small cell lung cancer (NSCLC). The major biological responses of TGF beta signaling are determined by the effector proteins SMAD2 and SMAD3. However, the regulators of TGF beta-SMAD signaling are not completely revealed yet. Here, we showed that the scaffolding protein PDLIM5 (PDZ and LIM domain protein 5, ENH) critically promotes TGF beta signaling by maintaining SMAD3 stability in NSCLC. First, PDLIM5 was highly expressed in NSCLC compared with that in adjacent normal tissues, and high PDLIM5 expression was associated with poor outcome. Knockdown of PDLIM5 in NSCLC cells decreased migration and invasionin vitroand lung metastasisin vivo. In addition, TGF beta signaling and TGF beta-induced epithelial-mesenchymal transition was repressed by PDLIM5 knockdown. Mechanistically, PDLIM5 knockdown resulted in a reduction of SMAD3 protein levels. Overexpression of SMAD3 reversed the TGF beta-signaling-repressing and anti-migration effects induced by PDLIM5 knockdown. Notably, PDLIM5 interacted with SMAD3 but not SMAD2 and competitively suppressed the interaction between SMAD3 and its E3 ubiquitin ligase STUB1. Therefore, PDLIM5 protected SMAD3 from STUB1-mediated proteasome degradation. STUB1 knockdown restored SMAD3 protein levels, cell migration, and invasion in PDLIM5-knockdown cells. Collectively, our findings indicate that PDLIM5 is a novel regulator of basal SMAD3 stability, with implications for controlling TGF beta signaling and NSCLC progression.
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