In-cell infrared difference spectroscopy of LOV photoreceptors reveals structural responses to light altered in living cells

Proteins are usually studied in well-defined buffer conditions, which differ substantially from those within a host cell. In some cases, the intracellular environment has an impact on the mechanism, which might be missed byin vitroexperiments. IR difference spectroscopy previously has been applied to study the light-induced response of photoreceptors and photoenzymesin vitro. Here, we established the in-cell IR difference (ICIRD) spectroscopy in the transmission and attenuated total reflection configuration to investigate the light-induced response of soluble proteins in living bacterial cells. ICIRD spectroscopy on the light, oxygen, or voltage (LOV) domains of the blue light receptors aureochrome and phototropin revealed a suppression of the response of specific secondary structure elements, indicating that the intracellular environment affects LOV photoreceptor mechanisms in general. Moreover, in-cell fluorescence spectroscopy disclosed that the intracellular environment slows down the recovery of the light-induced flavin adduct. Segment-resolved ICIRD spectroscopy on basic-region leucine zipper (bZIP)-LOV of aureochrome 1a from the diatomPhaeodactylum tricornutumindicated a signal progression from the LOV sensor to the bZIP effector independent of unfolding of the connecting A 'alpha-helix, an observation that stood in contrast toin vitroresults. This deviation was recapitulatedin vitroby emulating the intracellular environment through the addition of the crowding agent BSA, but not by sucrose polymers. We conclude that ICIRD spectroscopy is a noninvasive, label-free approach for assessing conformational changes in receptors in living cells at ambient conditions. As demonstrated, these near-native responses may deviate from the mechanisms established underin vitroconditions.