期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 129, 期 5, 页码 1287-1296出版社
OXFORD UNIV PRESS
DOI: 10.1111/jam.14675
关键词
amylomaltase; disproportionation reaction; GRAS; Saccharomyces cerevisiae; starch modification; Thermus filiformis
资金
- Rachadaphiseksomphot Fund of the Graduate School of Chulalongkorn University
- Centennial Academic Development Project
- Thailand Research Fund [IRG 5780008]
Aim To express amylomaltase from Thermus filiformis (TfAM) in a generally recognized as safe (GRAS) organism and to use the enzyme in starch modification. Methods and Results TfAM was expressed in Saccharomyces cerevisiae, using 2% (w/v) galactose inducer under GAL1 promoter. The enzyme was thermostable with high disproportionation and cyclization activities. The main large-ring cyclodextrin (CD) products were CD24-CD29, with CD26 as maximum at all incubation times. TfAM was used to modify cassava and pea starches, the amylose content decreased 18% and 30%, respectively, when 5% (w/v) starch was treated with 0 center dot 5 U TfAM g(-1) starch. The increase in short branched chain (DP, degree of polymerization, 1-5) and the broader chain length distribution pattern which extended to the longer chain (DP40) after TfAM treatment were observed. The thermal property was changed, with an increase in retrogradation of starch as suggested by a lower enthalpy. Conclusions TfAM was successfully expressed in S. cerevisiae and was used to make starches with new functionality. Significance and Impact of the Study This is the first report on the expression of AM in the GRAS yeast and the production of a modified starch gel from pea starch to improve the versatility of starch for food use.
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