期刊
JOURNAL OF AOAC INTERNATIONAL
卷 104, 期 1, 页码 223-231出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jaoacint/qsaa106
关键词
-
资金
- Ministry of Science, Technology and Telecommunications (MICITT)
- Innovation and Human Capital Program for Competitiveness (PINN)
- National Council for Scientific and Technology Research (CONICIT) at Costa Rica [PED-056-2015-I]
- NIH thought the University of Wisconsin, Department of Chemistry, Mass Spectrometry facility [NCRR 1S10RR024601-01]
The study identified the presence of A-type proanthocyanidins in cranberry products. Techniques like MALDI-TOF MS, deconvolution, and PCA were used to differentiate between A-type and B-type proanthocyanidins in cranberry-based foods and dietary supplements.
Background: Cranberry proanthocyanidins (c-PAC) are oligomeric structures of flavan-3-ol units, which possess A-type interflavan bonds. c-PAC differs from other botanical sources because other PAC mostly have B-type interflavan bonds. Cranberry products used to alleviate and prevent urinary tract infections may suffer from adulteration, where c-PAC are replaced with less expensive botanical sources of PAC that contain B-type interflavan bonds. Objective: Identifying the presence of A-type interflavan bonds in cranberry fruit and dietary supplements. Methods: Thirty-five samples reported to contain A-type PAC (cranberry fruit and cranberry products) and 36 samples reported to contain B-type PAC (other botanical sources) were identified and differentiated using MALDI-TOF MS, deconvolution of overlapping isotope patterns, and principal component analysis (PCA). Results: Our results show that both MALDI-TOF MS and deconvolution of overlapping isotope patterns were able to identify the presence of A-type interflavan bonds with a probability greater than 90% and a confidence of 95%. Deconvolution of MALDI-TOF MS spectra also determined the ratio of A-type to B-type interflavan bonds at each degree of polymerization in cranberry fruit and cranberry products, which is a distinguishing feature of c-PAC in comparison to other botanical sources of PAC. PCA shows clear differences based on the nature of the interflavan bonds. Conclusions: MALDI-TOF MS, deconvolution of overlapping isotope patterns of MALDI-TOF MS spectra, and PCA allow the identification, estimation, and differentiation of A-type interflavan bonds in cranberry-based foods and dietary supplements among other botanical sources containing mostly B-type interflavan bonds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据