Actinomadura decatromicinisp. nov., isolated from mountain soil in Thailand

A novel actinomycete strain CYP1-5(T)was isolated from the mountain soil sample collected from Chaiyaphum province, Thailand and its taxonomic position was clarified by using a polyphasic taxonomic approach. The chemotaxonomic properties of strain CYP1-5(T)were consistent within the genusActinomadura. Cell-wall peptidoglycan of this strain containedmeso-diaminopimelic acid. Galactose, madurose, and ribose were presented as the diagnostic sugars in whole-cell hydrolysates. The major menaquinone was MK-9(H-6). Major cellular fatty acids wereiso-C(16:0)and C-16:0. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, and phosphatidylinositol mannoside were observed as predominant phospholipids. Based on the results of phylogenetic analyses of 16S rRNA gene sequence, strain CYP1-5(T)was constituent with the genusActinomaduraand was closely related toActinomadura syzygiiGKU157(T)(99.5%) andActinomadura chibensisIFM 10266(T)(= JCM 14158(T)) (98.2%). The draft genome size of strain CYP1-5(T)was 9.30 Mb with 72.2 mol% of G + C content. Strain CYP1-5(T)showed ANIb values of 94.9% withA. syzygiiGKU157(T)and 93.2% withA. chibensisJCM 14158(T). Phenotypic characteristics, phylogenetic analysis and genome data support that strain CYP1-5(T)could be discriminated from its closest relatives, representing a novel species of the genusActinomadura, for which the nameActinomadura decatromicinisp. nov. is proposed. The type strain is CYP1-5(T)(= JCM 16996(T) = KCTC 19916(T) = TISTR 2901(T)).

Automated summary

A novel actinomycete strain CYP1-5(T) was isolated from mountain soil in Chaiyaphum province, Thailand. Through a polyphasic taxonomic approach, it was identified as a new species of the genus Actinomadura. Phylogenetic analysis and genome data supported the designation of this strain as Actinomadura decatromicina sp. nov.