期刊
JOURNAL DE MYCOLOGIE MEDICALE
卷 30, 期 2, 页码 -出版社
MASSON EDITEUR
DOI: 10.1016/j.mycmed.2020.100965
关键词
Cryptococcosis; Oxidative stress; Mitochondrial depolarization; Apoptosis; Anti-infection
类别
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [407192/2018-2]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE) [APQ-0108-2.08/14, APQ-0661-2.08/15]
- Fundacao de Amparo a Pesquisa e Desenvolvimento Cientifico do Maranhao [UNIVERSAL-00998/16, COOPI-02860/16]
Cryptococcosis is an opportunistic systemic mycosis whose treatment is limited to three drugs. In this work, we evaluated the antifungal activity of a hexane extract (HE) from Spondias tuberosa leaves against Cryptococcus neoformans and Cryptococcus gattii. Minimal inhibitory concentrations (MIC) were determined, and putative mechanisms were evaluated by flow cytometry. In addition, an in vivo infection assay was performed using Tenebrio molitor larvae. Treatment with HE inhibited the growth of standard and clinical isolates of C. neoformans and C. gattii (MICs ranging from 0.78 to 3.12 mg/mL), significantly (P < 0.05) increased mitochondrial superoxide anion levels, and induced mitochondrial membrane depolarization, loss of lysosomal membrane integrity, and phosphatidylserine externalization. The mean survival time of C. gattii-infected T. molitor larvae significantly (P < 0.05) increased from 1.225 days in control to 3.067 and 3.882 days in HE-treated groups (78 and 156 mg/kg, respectively). In conclusion, HE showed anticryptococcal activity, induced mitochondrial and lysosomal damage in yeast cells, and exhibited anti-infective action against C. gattii in T. molitor larvae. (C) 2020 Elsevier Masson SAS. All rights reserved.
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