The Genome Analysis ofMethylobacterium populiYC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase

Methylobacterium populiYC-XJ1 isolated from desert soil exhibited a diverse degrading ability towards aromatic oxyphenoxypropionic acid esters (AOPPs) herbicide, phthalate esters (PAEs), organophosphorus flame retardants (OPFRs), chlorpyrifos and phoxim. The genome of YC-XJ1 was sequenced and analyzed systematically. YC-XJ1 contained a large number of exogenous compounds degradation pathways and hydrolase resources. The quizalofop-p-ethyl (QPE) degrading geneqpeh2and diethyl phthalate (DEP) degrading genedeph1were cloned and expressed. The characteristics of corresponding hydrolases were investigated. The specific activity of recombinant QPEH2 was 0.1 +/- 0.02 U mg(-1)for QPE withk(cat)/K(m)values of 1.8 +/- 0.016 (mM(-1)center dot s(-1)). The specific activity of recombinant DEPH1 was 0.1 +/- 0.02 U mg(-1)for DEP withk(cat)/K(m)values of 0.8 +/- 0.02 (mM(-)(1)center dot s(-)(1)). This work systematically illuminated the metabolic versatility of strain YC-XJ1 via the combination of genomics analysis and laboratory experiments. These results suggested that strain YC-XJ1 with diverse xenobiotics biodegrading capacity was a promising candidate for the bioremediation of polluted sites.