期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 21, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/ijms21155289
关键词
Streptococcus suis; meningitis; DNase; neutrophils; NETs; pathogenesis
资金
- German Research Foundation [Deutsche Forschungsgemeinschaft e.V. (DFG)] of the Hannover Graduate School for Veterinary Pathobiology, Neuroinfectiology, and Translational Medicine (HGNI) [KO 3552/7-1]
- Akademie fur Tiergesundheit
- Ministry of Science and Culture of Lower Saxony (Georg-Christoph-Lichtenberg Scholarship) of the Hannover Graduate School for Veterinary Pathobiology, Neuroinfectiology, and Translational Medicine (HGNI)
- Niedersachsen-Research Network on Neuroinfectiology (N-RENNT) of the Ministry of Science and Culture of Lower Saxony DFG (Deutsche Forschungsgemeinschaft e.V.) [DFG KO 3552/7-1, DFG BA 4730/4-1]
- DFG
- University of Veterinary Medicine, Hannover, Foundation
Streptococcus suisis a zoonotic agent causing meningitis in pigs and humans. Neutrophils, as the first line of defense againstS. suisinfections, release neutrophil extracellular traps (NETs) to entrap pathogens. In this study, we investigated the role of the secreted nuclease A ofS. suis(SsnA) as a NET-evasion factor in vivo and in vitro. Piglets were intranasally infected withS. suisstrain 10 or an isogenicssnAmutant. DNase and NET-formation were analyzed in cerebrospinal fluid (CSF) and brain tissue. Animals infected withS. suisstrain 10 orS. suis10 Delta ssnAshowed the presence of NETs in CSF and developed similar clinical signs. Therefore, SsnA does not seem to be a crucial virulence factor that contributes to the development of meningitis in pigs. Importantly, DNase activity was detectable in the CSF of both infection groups, indicating that host nucleases, in contrast to bacterial nuclease SsnA, may play a major role during the onset of meningitis. The effect of DNase 1 on neutrophil functions was further analyzed in a 3D-cell culture model of the porcine blood-CSF barrier. We found that DNase 1 partially contributes to enhanced killing ofS. suisby neutrophils, especially when plasma is present. In summary, host nucleases may partially contribute to efficient innate immune response in the CSF.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据