期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
卷 32, 期 3, 页码 616-627出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09603123.2020.1789946
关键词
Green nanotechnology; cyanobacteria; silver oxide nanoparticles; gold nanoparticles; antiviral
In this study, biological nanoparticles were used as therapeutic antiviral agents and mediated by green Ag2O|AgO-NPs and Au-NPs for the treatment of Herpes Simplex virus (HSV-1). The results showed that both Ag2O|AgO-NPs and Au-NPs were able to reduce the cytopathic effect of HSV-1, with a higher reduction rate observed with Ag2O|AgO-NPs.
As therapeutic antiviral agents, biological nanoparticles can fight the drug-resistant types of viruses helping the antiviral drug development. In this study, two blue-green algal strains;Oscillatoriasp. andSpirulina platensiswere used, mediated by green Ag2O|AgO-NPs and Au-NPs, respectively. For NPs characterization, the UV/Vis spectroscopy were used where their formation and crystallinity were proven with lambda(max)values for silver and gold NPs of 432 and 552 nm, respectively. The transmission electron microscope (TEM) X-ray diffraction showed a spherical-shaped Ag2O|AgO-NPs (size; 14.42 to 48.97) while Au-NPs appeared with octahedral, pentagonal and triangular structures (size; 15.60-77.13 nm). The reducing, capping, and stabilization activities of algal polysaccharides and proteins were indicated via FTIR spectroscopy. Both Ag2O|AgO-NPs and Au-NPs were investigated against Herpes Simplex virus (HSV-1) that has been indicated by its reduction activity of cytopathic effect (CPE). Cytotoxicity was evaluated on Vero cells and measured by MTT assay. Results showed a 90% reduction in CPE of HSV-1 applying Ag2O|AgO-NPs, and Au-NPs at 31.25 mu L., with a high reduction rate (49.23%) with Ag2O|AgO-NPs than that of Au-NPs (42.75%). Current results proved the efficiency of green nanotechnology application with both Ag2O|AgO-NPs, and Au-NPs as reducing and inhibitory agents for the HSV-1 replication.
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