期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
卷 102, 期 16, 页码 4652-4663出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/03067319.2020.1786549
关键词
Escherichia coli; immunobiosensor; biological safety; bathing water
资金
- Estonian Research Council [IUT 20-17]
- Graduate School of Functional Materials and Technologies from the European Regional Development Fund in the University of Tartu, Estonia
Escherichia coli is commonly used as an indicator for water safety. Microbiological culture-based tests are the current 'gold' standard for E. coli detection, but they take a long time. Immunobiosensors offer a faster option for detecting E. coli and issuing timely warnings for water safety.
Escherichia coli is a Gram-negative, facultative anaerobic, rod-shaped coliform bacterium which is widely used as an indicator of biological safety of water. Currently, 'gold' standard for the detection ofE. coli(and other pathogens) are microbiological culture-based tests. Microbiological tests are very robust for the detection of live pathogens however it can take several days to get the results. A prospective option for the detection ofE. coliin recreational waters in addition to the genetic PCR analyses can be an immunobiosensor, allowing to detectE. coliin 20 min and to issue timely warnings of the safety of water. To compare different methods - microbiological culturing, quantitative PCR analysis and antibody-based immunobiosensor for the analysis of bathing water samples from a popular urban beach Anne Canal in Tartu, Estonia were used. The median value of biosensor results forE. coliwas considerably higher than the results of microbiological cultivation and qPCR: for 40 times, and 4 times, respectively. In addition, the biosensor results were in significant correlation with the number of total coliforms. Considering the current requirements for the biological safety of bathing water, we propose that the indicative safety threshold for bathing water usingimmunosensor analyses is in the range 4 x 10(3)-4 x 10(4)E. colicells/100 mL.
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