期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 164, 期 -, 页码 1640-1648出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.08.004
关键词
Artificial extracellular matrix; Elastin-like polymer; Enzymatic biodegradation
资金
- European Social Fund (ESF)
- European Regional Development Fund (ERDF)
- EU [H2020-NMP-2014-646075]
- JCyL [VA317P18]
- CIBERBBN
- Instituto de Salud Carlos III under the Network Center of Regenerative Medicine and Cellular Therapy of Castilla and Leon
- MICIUN [RTI2018-096320-B-C22, DTS19/00162, MAT2016-79435R, MAT2016-78903-R]
One of the main goals in both tissue engineering and regenerative medicine is to design innovative synthetic scaffolds that can simulate and control the communication pathways between cells and the extracellular matrix (ECM). In this context, we describe herein the characterization of protein polymer, a recombinant elastin-like recombinamer (ELR) designed for developing tissue-engineered devices for use in vascular regeneration. This ELR is composed of an elastin-like backbone that contains a fibronectin domain, which provides specific, endothelial cell adhesion, and a protease target domain directed towards specific proteases involved in ECM remodeling. We also compare the specific response of endothelial and fibroblast cells to ELR scaffolds and show that cell adhesion and spreading on this ELR is significantly higher for endothelial cells than for fibroblasts. The reactivity of this polymer and its hydrogels to specific enzymatic degradation is demonstrated in vitro. As with natural elastin, enzymatic hydrolysis of the ELR produces elastin-derived peptides, or matrikines, which, in turn, are potentially able to regulate important cell activities. (C) 2020 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据