期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 154, 期 -, 页码 267-275出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.03.104
关键词
Sweet cherry; Fruit softening; Sodium carbonate-soluble pectin (SSP); Pectin methyl esterase (PME); Gene expression; Chitosan
资金
- National Natural Science Foundation of China [31601519, 21676073]
- National key research and development program in 13th Five-Year of China [2018YFD0401102]
- Natural Science Foundation of Jiangsu Province [BK20181184]
- Singapore Ministry of Education [R-160-000A40-114]
The inhibiting effect of chitosan coating (2%) on the softening and sodium carbonate-soluble pectin (SSP) evolution of sweet cherries during non-isothermal storage was investigated. Chitosan coating significantly extend the softening (6.4% greater than the control group), maintained the SSP content (6.6% greater than the control group), and reduced the degradation of SSP by inhibiting the expression of the paPME1-5 genes, which regulating pectin methylesterase activity of sweet cherries under temperature variation. In addition, the results of methylation and monosaccharide composition indicated that the chitosan coating reduced demethylation of SSP and the loss of RG-I main and side chain neutral sugars. Atomic force microscopy images revealed that the coated sweet cherries contained more linked, branched, and long SSP chains and maintained the width of the pectin backbone (>140 nm). These results indicated that a chitosan coating is feasible to preserve postharvest fruit under non-isothermal conditions. (C) 2020 Elsevier B.V. All rights reserved.
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