Evaluation of structure transformation and biocompatibility of chitosan in alkali/urea dissolution system for its large-scale application

Alkali/urea, a green dissolution system for chitosan, have been widely used in preparation of chitosan-derived materials. However, there are no related reports detail the structure stability and biocompatibility of chitosan in alkali/urea, which are important for its large-scale application. In this work, chitosan was dissolved in KOH/urea solution and stored at different temperature for different time. The structure, viscosity, molecule weight (Mw), degree of deacetylation (DD), and biocompatibility of chitosan were determined. The Mw of chitosan decreased from 1011 KDa to 827-834 KDa, and DD increased from 76.9% to 85.7-93.5% after been stored at 25 degrees C and 4 degrees C for 5 weeks. Incomplete dissolution of chitosan and increase of DD enhanced its thermal stability by forming new crystallization zone. In contrast, chitosan stored in-20 degrees C for 5 weeks was stable without obvious change of Mw and DD. Moreover, the processed chitosans were no-toxic and safety for the biomedicine applications. (C) 2020 Elsevier B.V. All rights reserved.