期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 156, 期 -, 页码 354-361出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.04.024
关键词
Bacillus velezensis SN-1; Capsular polysaccharides (CPS); Antioxidant activity in vitro; Antitumor activity in vitro
资金
- National Natural Science Foundation of China [31972047]
- Guiding Plan of Natural Science Foundation of Liaoning Province [2019-ZD-0714]
- Liaoning Revitalization Talents Program [XLYC1807040]
- Liaoning Support Programfor innovative talents [LR2019065]
- Liaoning BaiQianWan Talents Program Project
A capsular polysaccharides (CPS) producer Bacillus velezensis SN-1 (B. velezensis SN-1) was isolated in Da-Jiang, China. We used ultrasonic extraction to obtain CPS from a culture of B. velezensis SN-1 at a yield of 755 mg/L. Using gel permeation chromatography (GPC), CPS was separated into a single peak with a molecular weight of 1.46 x 10(5) Da. Its structures were characterized by gas chromatography (GC), methylation, Fourier transform infrared spectroscopy (MR), and nuclear magnetic resonance (NMR). The CPS was identified as a polysaccharide with a highly branched main chain of mannose with (1 -> 3) connections. Moreover, our results revealed that CPS has the capacity to scavenge DPPH radical, hydroxyl radical, ABTS radical and oxygen radical in a manner that relied on concentration. Anti-neoplastic analysis showed that CPS displayed significant anti-tumor activity towards HepG-2 tumor cells. Above findings indicate that CPS generated by B. velezensis SN-1 may be adapted for use as a natural antioxidant in foodstuffs and as an anti-tumor drug. (C) 2020 Elsevier B.V. All rights reserved.
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