期刊
CLINICAL AND EXPERIMENTAL ALLERGY
卷 46, 期 2, 页码 365-376出版社
WILEY
DOI: 10.1111/cea.12680
关键词
allergen; Der p 23; house dust mite; RNA-seq
资金
- National Institute of Environmental Health Sciences, National Institutes of Health [Z01-ES102885-01, ZIA-ES102645]
- National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R01AI077653]
BackgroundDer p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein. ObjectiveThis study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population and to determine the structure for functional characterization. MethodsIgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7 and Der p 8 was measured by ELISA. RNA-seq data from D.pteronyssinus were compared as estimates of allergen expression levels. The structure was analysed by X-ray crystallography and NMR. ResultsDespite a high prevalence of Der p 23, (75% vs. 87% and 79% for Der p 1 and Der p 2, respectively), the anti-Der p 23 IgE levels were relatively low. The patient response to the 6 allergens tested was variable (n=47), but on average anti-Der p 1 and anti-Der p 2 together accounted for 85% of the specific IgE. In terms of abundance, the RNA expression level of Der p 23 is the lowest of the major allergens, thirty fold less than Der p 1 and sevenfold less than Der p 2. The structure of Der p 23 is a small, globular protein stabilized by two disulphide bonds, which is structurally related to allergens such as Blo t 12 that contain carbohydrate binding domains that bind chitin. Functional assays failed to confirm chitin binding by Der p 23. Conclusions and Clinical RelevanceDer p 23 accounts for a small percentage of the IgE response to mite allergens, which is dominated by Der p 1 and Der p 2. The prevalence and amount of specific IgE to Der p 23 and Der p 2 are disproportionately high compared to the expression of other Dermatophagoides allergens.
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