期刊
GENES TO CELLS
卷 25, 期 9, 页码 615-625出版社
WILEY
DOI: 10.1111/gtc.12797
关键词
Chikungunya virus; molecular diagnosis; RT-LAMP
资金
- Japan Society for the Promotion of Science [17K08889, 17H04649, 18K16155, 17K17104, 17K15680]
- Suzuken Memorial Foundation
- Kurozumi Medical Foundation
- GSK Japan Research Grant 2016
- Grants-in-Aid for Scientific Research [17H04649, 17K15680, 17K17104, 17K08889, 18K16155] Funding Source: KAKEN
Chikungunya fever is a mosquito-borne disease cause of persistent arthralgia. The current diagnosis of Chikungunya virus (CHIKV) relies on a conventional reverse transcription polymerase chain reaction assay. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid and simple tool used for DNA-based diagnosis of a variety of infectious diseases. In this study, we established an RT-LAMP system to recognize CHIKV by targeting the envelope protein 1 (E1) gene that could also detect CHIKV at a concentration of 8 PFU without incorrectly detecting other mosquito-borne viruses. The system also amplified the E1 genome in the serum of CHIKV-infected mice with high sensitivity and specificity. Moreover, we established a dry RT-LAMP system that can be transported without a cold chain, which detected the virus genome in CHIKV-infected patient samples with high accuracy. Thus, the dry RT-LAMP system has great potential to be applied as a novel CHIKV screening kit in endemic areas.
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