期刊
FREE RADICAL RESEARCH
卷 54, 期 11-12, 页码 918-930出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715762.2020.1791843
关键词
Autophagy; neurodegenerative diseases; heme oxygenase-1; Alzheimer's Disease; Parkinson's Disease
资金
- Priority Research Centres Programme through the National Research Foundation of Korea (NRF) - Ministry of Education [2014R1A6A1030318, NRF-2019R1I1A3A01058874, NRF-2017R1A6A3A11031089]
- National Natural Science Foundation of China [81460212]
The translocation of transcription factor EB (TFEB) to the nucleus plays a pivotal role in the regulation of basic cellular processes, such as lysosome biogenesis and autophagy. Autophagy is an intracellular degradation system that delivers cytoplasmic constituents to the lysosome, which is important in maintaining cellular homeostasis during environmental stress. Furthermore, oxidative stress is a critical cause for the progression of neurodegenerative diseases. Curcumin has anti-oxidative and anti-inflammatory activities, and is expected to have potential therapeutic effects in various diseases. In this study, we demonstrated that curcumin regulated TFEB export signallingviainhibition of glycogen synthase kinase-3 beta (GSK-3 beta); GSK-3 beta was inactivated by curcumin, leading to reduced phosphorylation of TFEB. We further showed that H2O2-induced oxidative stress was reduced by curcuminviathe Nrf2/HO-1 pathway in human neuroblastoma cells. In addition, we showed that curcumin induced the degradation of amyloidogenic proteins, including amyloid-beta precursor protein and alpha-synuclein, through the TFEB-autophagy/lysosomal pathway. In conclusion, curcumin regulates autophagy by controlling TFEB through the inhibition of GSK-3 beta, and increases antioxidant gene expression in human neuroblastoma cells. These results contribute to the development of novel cellular therapies for neurodegenerative diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据