期刊
FOOD RESEARCH INTERNATIONAL
卷 134, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.foodres.2020.109226
关键词
Anthocyanin derivatives; Triple-quadrupole mass spectrometry; Ion trap mass spectrometry; Method validation; Partial least squares discriminant analysis
资金
- National Natural Science Foundation of China [31471723]
- China Agriculture Research System for Grape Industry [CARS-29]
Anthocyanin derivatives are critical components that impart color to aging red wine. In this study, we developed a targeted metabolomic method for the simultaneously profiling of the primary thirty-seven malvidin-derived anthocyanin derivatives in red wine, including various pyranoanthocyanins and flavanols-related condensation products. First, high-performance liquid chromatography (HPLC) tandem ion trap and triple-quadrupole (QqQ) mass spectrometry were used to construct the mass spectral and chromatographic database of the anthocyanin derivatives that were formed in a model wine solution. Next, the targeted profiling analysis of these compounds was achieved on a QqQ mass spectrometer in the multiple reaction monitoring mode (MRM). The method displayed excellent linearity (R-2 0.9391-0.9998), sensitivity (0.221-0.604 mu g/L of limit of detection (LOD) and 0.274-1.157 mu g/L of limit of quantification (LOQ) equivalent to malvidin-3-O-glucoside (Mv-glc)), and repeatability (less than 10% and 15% for intra-day and inter-day relative standard deviation (RSD) respectively). Partial least squares discriminant analysis (PLS-DA) based on this method showed great discrimination over different vintage wines, thereby promising to be an effective tool in wine anthocyanin and aging related study.
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