期刊
FOOD HYDROCOLLOIDS
卷 105, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2020.105802
关键词
Sugar beet pectin; Genipin; Crosslinking; Emulsifying stability
资金
- National Natural Science Foundation of China [31601423, 31771931]
- China Postdoctoral Science Foundation [2019M653279]
- 111 Project [B17018]
This work aimed to construct conjugates of sugar beet pectin (SBP) with exogenous proteins by adopting a genipin (GP) crosslinking strategy. The conversion processes of two protein, bovine serum albumin (BSA) and whey protein isolate (WPI) to the molecular chains of SBP were investigated using high-performance sizeexclusion chromatography technology under varying SBP/exogenous protein weight ratios (SPR) (2:1-20:1) and crosslinking times (1-25 h). When prepared at SPR 10:1, the formed SBP-WPI/BSA-SBP conjugates had notably higher molecular weights of 2151. 68 kg/mol and 1758. 45 kg/mol, respectively, in comparison with that of SBP (289. 16 kg/mol). In terms of reactivity, WPI showed higher reactivity than BSA in crosslinking with SBP by GP. Two representative samples, SBP-WPI-Conjugate and SBP-BSA-Conjugate, prepared at SPR 10:1 after 25 h of crosslinking were further analyzed for surface hydrophobicity (S-0) and emulsifying properties. It was found that S-0 values of the SBP-WPI-Conjugate and SBP-BSA-Conjugate were significantly lower than that of the control blend samples. Furthermore, the selected conjugates showed superior emulsion stabilizing properties as compared with SBP-exogenous protein blends or individual SBP and exogenous proteins. After storage at 60 degrees C for 7 d, emulsions stabilized by SBP-WPI-Conjugate and SBP-BSA-Conjugate at concentration of 1 g/100 g emulsion had mean droplet diameters of 0.735. 0.023 mu m and 0.733. 0.011 mu m at pH 3.5, and 0.972. 0.020 mu m and 1.07. 0.08 mu m at pH 7.0, respectively. Altogether, the work demonstrates that the GP crosslinking method is suitable for upgrading SBP to SBP-protein conjugate with enhanced emulsification properties.
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