期刊
FOOD CHEMISTRY
卷 315, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.126310
关键词
Lateral flow immunoassay; Furazolidone; Polydopamine nanospheres; Binding stability
资金
- National Natural Science Foundation of China [21675127]
- Shaanxi Provincial Science Fund for Distinguished Young Scholars [2018JC-011]
- Key Research and Development Plan of Shaanxi Province [2019NY-109]
- Development Project of Qinghai Provincial Key Laboratory [2017-ZJ-Y10]
- Capacity Building Project of Engineering Research Center of Qinghai Province [2017-GX-G03]
Currently, the low sensitivity and poor binding stability of detection probe prepared via electrostatic adsorption have become the dilemmas of colloidal gold-based lateral flow immunoassays (Au-LFIAs). In this connection, polydopamine nanospheres (PDA NPs) with an eminent covalent connectivity property were introduced as a promising substitute to improve the stability of probe and sensitivity of LFIA. Whereafter, the PDA NPs-based LFIA was applied for the monitoring of furazolidone (FZD) in food samples because of the potential carcinogenic/mutagenic effects to human of its metabolite (3-amino-2-oxazolidinone, AOZ). Compared with electrostatic adsorption, the binding stability of PDA NPs-based probes was superior. And, as expected, the PDA NPs-based LFIA biosensor exhibited higher sensitivity than that of the Au-LFIA with a detection limit of 3.5 ng mL(-1) for AOZ by naked-eye readout. Based on the significant enhanced binding stability and sensitivity, the PDA NPsbased LFIA is of certain spreading value for detecting other analytes.
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