期刊
FOOD CHEMISTRY
卷 320, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.126647
关键词
Okra (Abelmoschus esculentus (L.) Moench); Polysaccharide; Preparation; Characterization; Bioactivity
资金
- National Natural Science Foundation of China [31671812]
- Key Research & Development Program (Modern Agriculture) of Jiangsu Province [BE2017362]
- Policy Guidance Projects in Jiangsu Province (Science and technology special project in Northern Jiangsu Province) [SZ-SQ2019038]
- Key Research & Development Program (Modern Agriculture) of Sihong County [L201803]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
- Jiangsu Overseas Research & Training Program for University Prominent Young & Middle-aged Teachers and Presidents
In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)(2)SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods. Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)(2)SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)(2)SO4, respectively. OPS-50 had lower weight-average molecular weight (3.65 x 10(6) g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity. Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and alpha-amylase and alpha-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.
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