期刊
FISH & SHELLFISH IMMUNOLOGY
卷 106, 期 -, 页码 691-704出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2020.07.019
关键词
Turbot Scophthalmus maximus; Myo-inositol; Expression tendency analysis; WGCNA; Integrated transcriptome analyses; Cytokine-cytokine receptor interaction
资金
- Earmarked Fund for Modern Agro-Industry Technology Research System [CARS-47-G01]
- Agricultural Fine Breed Project of Shandong [2019LZGC013]
- AoShan Talents Cultivation Program
- Qingdao National Laboratory for Marine Science and Technology [2017ASTCP-OS04]
- National Natural Science Foundation of China [41706168]
- Basic scientific research Fund of China Academy of Fishery Sciences [2020TD25]
- Yantai science and technology plan [2018ZDCX021]
Myo-inositol is an essential vitamin for most animals, and it can modulate multiple physiological functions. In this study, we performed transcriptome gene expression profiling of gill tissue from turbot Scophthalmus maximus fed different concentrations of myo-inositol (0, 300, 600, 900, 1200 mg/kg). Results of expression tendency analysis, Weighted Gene Co-Expression Network Analysis (WGCNA), integrated transcriptome analyses, and KEGG annotation analysis of all differentially expressed genes (DEGs) demonstrated that the cytokine-cytokine receptor interaction played a core role in effects of myo-inositol on turbot, which was followed by the Jak-STAT signaling pathway. The results of qRT-PCR also showed myo-inositol mediated the gene expression of the cytokine-cytokine receptor interaction and the Jak-STAT signaling pathway in turbot. The ELISA assay indicated that myo-inositol affected the concentration change of interleukins (IL-2 and IL-10). Consequently, the interleukins associated with immune functions in the cytokine-cytokine receptor interaction played a core role in the effects of myo-inositol on turbot, which was followed by the Jak-STAT signaling pathway. Additionally, 10 hub genes associated with myo-inositol-traits were identified via WGCNA.
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