期刊
FISH & SHELLFISH IMMUNOLOGY
卷 103, 期 -, 页码 229-238出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2020.05.002
关键词
Regucalcin; Apoptosis; Lipopolysaccharide stimulation; Immune response; Crassostrea gigas
资金
- National Key RD Program [2018YFD0900502]
- National Science Foundation of China [U1706204, 41961124009]
- Key R&D Program of Liaoning Province [2017203004, 2017203001]
- earmarked fund from Modern Agro-industry Technology Research System [CARS-49]
- Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research
- Distinguished Professor of Liaoning
- AoShan Talents Cultivation Program
- Qingdao National Laboratory for Marine Science and Technology [2017ASTCP-OS13]
- Dalian High Level Talent Innovation Support Program [2015R020]
- Research Foundation for Talented Scholars in Dalian Ocean University
Regucalcin (RGN), also known as senescence marker protein-30 (SMP30), plays a vital role in the regulation of Ca2+ homeostasis. In the present study, a regucalcin (designated as CgRGN) was identified from Pacific oyster Crassostrea gigas. The complete cDNA sequence of CgRGN was of 1059 bp, containing an open reading frame of 933 bp which encoded a protein of 310 amino acids. The deduced amino acid sequence of CgRGN shared similarity with other RGNs from the genome of C. gigas as well as other species. The mRNA transcripts of CgRGN were universally detected in all tested tissues, with higher level in hepatopancreas, labial palp, and gills. The relative expression level of CgRGN in hemocytes was significantly up-regulated (p < 0.05) at 3, 12, 72, and 96 h after the stimulation of lipopolysaccharide (LPS). After CgRGN expression was interfered by specific CgRGN-dsRNA, the hemocytes apoptosis rate increased dramatically at 12 h post LPS stimulation (1.56 fold, p < 0.01), compared to the control group. The caspase-3 activity in hemocytes and NO concentration in hemolymph increased significantly (p < 0.05) in dsCgRGN injection oysters. These results collectively indicated that CgRGN could suppress LPS-induced apoptosis and be involved in the immune response of oysters.
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