期刊
FEBS LETTERS
卷 594, 期 19, 页码 3122-3141出版社
WILEY
DOI: 10.1002/1873-3468.13888
关键词
acetate; autophagy; ChlamydomonasS6 kinase; lipid accumulation; N starvation; TOR kinase activity
资金
- JC Bose Fellowship Grant (DST) [10X-217]
- Department of Atomic Energy (Government of India) [12P0123]
Target of rapamycin (TOR) kinase is a sensor and a central integrator of internal and external metabolic cues. However, in algae and in higher plants, the components of TOR kinase signaling are yet to be characterized. Here, we establish an assay system to study TOR kinase activity inChlamydomonas reinhardtiiusing the phosphorylation status of its putative downstream target, CrS6K. Using this assay, we probe the modulation of cellular TOR kinase activity under various physiological states such as photoautotrophy, heterotrophy, mixotrophy, and nitrogen (N) starvation. Importantly, we uncover that excess acetate in the medium leads to high cellular reactive oxygen species levels, triggering autophagy and a concomitant drop in TOR kinase activity in a dose-dependent manner, thus leading to a N-starvation-like cellular phenotype, even when nitrogen is present.
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