期刊
FASEB JOURNAL
卷 34, 期 8, 页码 10212-10227出版社
WILEY
DOI: 10.1096/fj.201902569R
关键词
GCRV; innate immunity; prmt3; SVCV; zebrafish
资金
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA 24010308]
- National Natural Science Foundation of China [31721005, 31830101, 31671315]
- National Key RAMP
- D Program of China [2018YFD0900602]
Arginine methylation catalyzed by protein arginine methyltransferases (PRMT) is a common post-translational modification in histone and nonhistone proteins, which regulates many cellular functions. Protein arginine methyltransferase 3 (prmt3), a type I arginine methyltransferase, has been shown to carry out the formation of stable monomethylarginine as an intermediate before the establishment of asymmetric dimethylarginine. To date, however, the role of PRMT3 in antiviral innate immunity has not been elucidated. This study showed that zebrafishprmt3was upregulated by virus infection and that the overexpression ofprmt3suppressed cellular antiviral response. The PRMT3 inhibitor, SGC707, enhanced antiviral capability. Consistently,prmt3-null zebrafish were more resistant to Spring Viremia of Carp Virus (SVCV) and Grass Carp Reovirus (GCRV) infection. Further assays showed that the overexpression ofprmt3diminished the phosphorylation of irf3 and prmt3 interacted with rig-i. In addition, both zinc-finger domain and catalytic domain of prmt3 were required for the suppressive function ofprmt3on IFN activation. Our findings suggested that zebrafishprmt3negatively regulated the antiviral responses, implicating the vital role ofprmt3-or even arginine methylation-in antiviral innate immunity.
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