An Automatic Nucleus Segmentation and CNN Model based Classification Method of White Blood Cell

White blood cells (WBCs) play a remarkable role in the human immune system. To diagnose blood-related diseases, pathologists need to consider the characteristics of WBC. The characteristics of WBC can be defined based on the morphological properties of WBC nucleus. Therefore, nucleus segmentation plays a vital role to classify the WBC image and it is an important part of the medical diagnosis system. In this study, color space conversion and k-means algorithm based new WBC nucleus segmentation method is proposed. Then we localize the WBC based on the location of segmented nucleus to separate them from the entire blood smear image. To classify the localized WBC image, we propose a new convolutional neural network (CNN) model by combining the concept of fusing the features of first and last convolutional layers, and propagating the input image to the convolutional layer. We also use a dropout layer for preventing the model from overfitting problem. We show the effectiveness of our proposed nucleus segmentation method by evaluating with seven quality metrics and comparing with other methods on four public databases. We achieve average accuracy of 98.61% and more than 97% on each public database. We also evaluate our proposed CNN model by using nine classification metrics and achieve an overall accuracy of 96% on BCCD test database. To validate the generalization capability of our proposed CNN model, we show the training and testing accuracy and loss curves for random test set of BCCD database. Further, we compare the performance of our proposed CNN model with four state-of-the-art CNN models (biomedical image classifier) by measuring the value of evaluation metrics. (C) 2020 Elsevier Ltd. All rights reserved.