期刊
EUROPEAN JOURNAL OF NEUROSCIENCE
卷 53, 期 2, 页码 416-429出版社
WILEY
DOI: 10.1111/ejn.14925
关键词
ERK; Fos; Iba-1; MMP; NF-kappa B
资金
- National Institute of Mental Health [R25MH080661]
- National Institutes of Health [P30A1082151]
- Johns Hopkins University
Combined antiretroviral therapy for HIV infection can reduce plasma viral load and prolong life, but the brain remains a viral reservoir with pathologies such as cognitive decline and blood-brain barrier disruption. Methamphetamine abuse is common among HIV-infected individuals and both methamphetamine and HIV toxic proteins can disrupt the blood-brain barrier, potentially involving common pathways such as NF-kappa B/MMP-9 in inducing barrier damage.
Combined antiretroviral therapy for HIV infection reduces plasma viral load and prolongs life. However, the brain is a viral reservoir, and pathologies such as cognitive decline and blood-brain barrier (BBB) disruption persist. Methamphetamine abuse is prevalent among HIV-infected individuals. Methamphetamine and HIV toxic proteins can disrupt the BBB, but it is unclear if there exists a common pathway by which HIV proteins and methamphetamine induce BBB damage. Also unknown are the BBB effects imposed by chronic exposure to HIV proteins in the comorbid context of chronic methamphetamine abuse. To evaluate these scenarios, we trained HIV-1 transgenic (Tg) and non-Tg rats to self-administer methamphetamine using a 21-day paradigm that produced an equivalency dose range at the low end of the amounts self-titrated by humans. Markers of BBB integrity were measured for the hippocampus, a brain region involved in cognitive function. Outcomes revealed that tight junction proteins, claudin-5 and occludin, were reduced in Tg rats independent of methamphetamine, and this co-occurred with increased levels of lipopolysaccharide, albumin (indicating barrier breakdown) and matrix metalloproteinase-9 (MMP-9; indicating barrier matrix disruption); reductions in GFAP (indicating astrocytic dysfunction); and microglial activation (indicating inflammation). Evaluations of markers for two signaling pathways that regulate MMP-9 transcription, NF-kappa B and ERK/ increment FosB revealed an overall genotype effect for NF-kappa B. Methamphetamine did not alter measurements from Tg rats, but in non-Tg rats, methamphetamine reduced occludin and GFAP, and increased MMP-9 and NF-kappa B. Study outcomes suggest that BBB dysregulation resulting from chronic exposure to HIV-1 proteins or methamphetamine both involve NF-kappa B/MMP-9.
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