期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 50, 期 8, 页码 1234-1236出版社
WILEY
DOI: 10.1002/eji.202048715
关键词
antibodies; COVID-19; LIPS; luciferase immunoprecipitation; SARS-CoV-2
类别
资金
- Estonian Research Council [PRG377, PUT1367]
Profiling antibodies to SARS-CoV-2 can help to assess potential immune response after COVID-19 disease. Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS to detect antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 26 out of 26 COVID-19 patients with N antigen or with three protein fragments when combined into a single reaction. The assay correlated well with ELISA method and was specific to COVID-19 as we saw no reactivity among uninfected healthy controls. Our results show that LIPS is a rapid and measurable method to screen antibody responses against SARS-CoV-2 antigens.
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