期刊
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
卷 246, 期 9, 页码 1857-1865出版社
SPRINGER
DOI: 10.1007/s00217-020-03539-3
关键词
Bisphenol S (BPS); Monoclonal antibody (mAb); Chemiluminescence immunoassay (CLIA); Canned beverage; High-performance liquid chromatography-UV (HPLC-UV)
资金
- National Natural Science Foundation of China [31572556, 31873006]
- Key Program for International S&T Cooperation Project of Shaanxi Province [2017KW-ZD-10]
- Incubation Project on State Key Laboratory of Biological Resources and Ecological Environment of Qinba Areas [SLGPT2019KF04-04]
- FCT I.P
- Ministerio da Ciencia Tecnologia e Ensino Superior (MCTES)
- European Regional Development Fund (ERDF) [POCI-01-0145-FEDER-007569]
Bisphenol S (BPS) is a major alternative to Bisphenol A (BPA) newly applied in food packing material and disrupts reproductive and developmental endpoints through endocrine pathways. This study aimed to develop a rapid, sensitive, and accurate chemiluminescence immunoassay (CLIA) for the detection of BPS in canned beverage. Anti-BPS monoclonal antibody (mAb) was generated by immunizing Balb/c mice with immunogen (BPS-BSA conjugation). CLIA assay conditions, including 3-[9-(((3-(N-succinimidyloxycarboxypropyl) [4-methxylphenyl] sulfonyl) amine) carboxyl]-10-acridiniumyl)-1-propanesulfonate inner salt (NSP-SA-NHS) labeling ratio (1:40), antigen concentration (1 mu g mL(-1)) and antibody titer (1:8000; 125 ng mL(-1)in PBS (0.01 M, pH 7.4)) were determined, respectively. The half inhibition concentration (IC50), detection limit (IC10), and working range of the CLIA in canned beverage were 0.04, 1.48, and 0.32-5 ng mL(-1), respectively. Cross-reactivity with other bisphenols (4,4 '-thiodiphenol, Bisphenol F, 4,4 '-dihydroxy benzophenone, Bisphenol A and Bisphenol B) was less than 0.56%, and the recovery rates of BPS in canned beverage ranged from 80 to 109.2% (intra-assay) and from 82 to 108.4% (inter-assay). The results of CLIA were compared with high-performance liquid chromatography-UV (HPLC-UV) analysis, showing a good correlation of 0.956. After proper sample pre-treatment, this method allows relatively large amounts of sample analysis in a limited time period (30 min). Thus, it was deemed suitable and efficient for routine screening of BPS in canned beverage. [GRAPHICS] .
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