期刊
EMBO JOURNAL
卷 39, 期 17, 页码 -出版社
WILEY
DOI: 10.15252/embj.2019104337
关键词
genome-widede novoinsertion sites; integrase genome-wide occupancy; RNA Polymerases I and III; Ty1 retrotransposon
资金
- Centre National de la Recherche Scientifique (CNRS)
- Universite Paris Diderot
- Institut National de la Sante et de la Recherche Medicale (INSERM)
- Fondation ARC pour la Recherche sur le Cancer [PJA 20151203412]
- Agence Nationale de la Recherche [ANR-13-BSV3-0012, ANR-17-CE11-0025]
- Fondation pour la Recherche Medicale [FRM-SPF20170938755]
- ANR through the initiatives d'excellence [Idex ANR-11-IDEX-0005-02]
- ANR through Labex Who am I? [ANR-11-LABX-0071]
- PhD program from the CEA
- Agence Nationale de la Recherche (ANR) [ANR-11-LABX-0071, ANR-13-BSV3-0012] Funding Source: Agence Nationale de la Recherche (ANR)
Integration of transposable elements into the genome is mutagenic. Mechanisms targeting integrations into relatively safe locations, hence minimizing deleterious consequences for cell fitness, have emerged during evolution. In budding yeast, integration of the Ty1 LTR retrotransposon upstream of RNA polymerase III (Pol III)-transcribed genes requires interaction between Ty1 integrase (IN1) and AC40, a subunit common to Pol I and Pol III. Here, we identify the Ty1 targeting domain of IN1 that ensures (i) IN1 binding to Pol I and Pol III through AC40, (ii) IN1 genome-wide recruitment to Pol I- and Pol III-transcribed genes, and (iii) Ty1 integration only at Pol III-transcribed genes, while IN1 recruitment by AC40 is insufficient to target Ty1 integration into Pol I-transcribed genes. Swapping the targeting domains between Ty5 and Ty1 integrases causes Ty5 integration at Pol III-transcribed genes, indicating that the targeting domain of IN1 alone confers Ty1 integration site specificity.
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