期刊
EMBO JOURNAL
卷 39, 期 16, 页码 -出版社
WILEY
DOI: 10.15252/embj.2019103373
关键词
heterochromatin; mitosis; nuclear speckles; nucleolus; phase-transition
资金
- advanced ERC grant ChroNeuroRepair [340793]
- German Research Foundation
- NSFC [31730110]
TMF1-regulated nuclear protein 1 (Trnp1) has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding, but its molecular function in the nucleus is still unknown. Here, we show that Trnp1 is a low complexity protein with the capacity to phase separate. Trnp1 interacts with factors located in several nuclear membrane-less organelles, the nucleolus, nuclear speckles, and condensed chromatin. Importantly, Trnp1 co-regulates the architecture and function of these nuclear compartmentsin vitroand in the developing brainin vivo. Deletion of a highly conserved region in the N-terminal intrinsic disordered region abolishes the capacity of Trnp1 to regulate nucleoli and heterochromatin size, proliferation, and M-phase length; decreases the capacity to phase separate; and abrogates most of Trnp1 protein interactions. Thus, we identified Trnp1 as a novel regulator of several nuclear membrane-less compartments, a function important to maintain cells in a self-renewing proliferative state.
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