期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 97, 期 3, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2020.115059
关键词
Carbapenemase; OXA-244; OXA-48; Detection
资金
- University of Fribourg by the Swiss National Reference center for Emerging Antibiotic Resistance
- Swiss National Science Foundation [FNS-31003A_163432]
- Bundesministerium fuer Bildung und Forschung (BMBF, Germany) within the German Center for Infection Research (DZIF) [8032808820]
OXA-244 is a derivative of OXA-48 showing weaker carbapenemase activity, compromising the detection of corresponding producers in clinical laboratories. Since 2017, the Swiss National Reference Center for Emerging Antibiotic Resistance noticed an increased identification of OXA-244-producing Escherichia coli (n=15) within the country. Different methods (biochemical and immunoassay tests, screening culture media) were tested for the detection of OXA-244 producers. Whole genome sequencing was used to investigate the genetic relatedness between the isolates and the genetic structures at the origin of the acquisition of the bla(OXA-244) gene. The mSuperCARBA (R) medium and the NG-Test CARBA5 assay were found to be suitable tools for detecting all OXA-244-producing isolates.Other selective media did not perform optimally. Among the fifteen strains, live sequence types were identified, with ST38 being predominant. The bla(OXA-244) gene was located on the chromosome for all isolates. Overall, detection of OXA-244 producers is challenging and specific: guidelines must be followed. (C) 2020 The Authors. Published by Elsevier Inc.
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