期刊
DEVELOPMENTAL CELL
卷 54, 期 3, 页码 379-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2020.06.003
关键词
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资金
- E. Bayard Halsted Fellowship
- Schlumberger Faculty for the Future Fellowship
- HHMI International Student Research Fellowship
- NIH [R01-GM058921, R01-GM122936]
Many membraneless organelles form through liquid-liquid phase separation, but how their size is controlled and whether size is linked to function remain poorly understood. The histone locus body (HLB) is an evolutionarily conserved nuclear body that regulates the transcription and processing of histone mRNAs. Here, we show that Drosophila HLBs form through phase separation. During embryogenesis, the size of HLBs is controlled in a precise and dynamic manner that is dependent on the cell cycle and zygotic histone gene activation. Control of HLB growth is achieved by a mechanism integrating nascent mRNAs at the histone locus, which facilitates phase separation, and the nuclear concentration of the scaffold protein multi-sex combs (Mxc), which is controlled by the activity of cyclin-dependent kinases. Reduced Cdk2 activity results in smaller HLBs and the appearance of nascent, misprocessed histone mRNAs. Thus, our experiments identify a mechanism linking nuclear body growth and size with gene expression.
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